TABLE 4.
Formation of 13C-labeled fermentation products during [1-13C]glucose consumption at 30°C by high-density suspensions of nongrowing L. lactis cells
| L. lactis strain | [13C]glucose consumed (%) | Amt of product (mol mol of [1-13C]glucose−1)a
|
||||||
|---|---|---|---|---|---|---|---|---|
| Lactate | Acetate | Ethanol | Acetoin | 2,3-Butanediol | Mannitol | 13C recovery | ||
| NZ9000 | 70 | 0.68 | 0.68 | |||||
| NZ9000/pNZmt1D | 85 | 0.62 | 0.02 | 0.64 | ||||
| NZ9010 | 44 | 0.17 | 0.22 | 0.24 | 0.02 | 0.65 | ||
| NZ9010/pNZmt1D | 100 | 0.07 (0.3) | (0.14) | 0.03 (0.11) | 0.14 (0.22) | 0.07 (0.15) | 0.15 (0.25) | 0.46 (0.90) |
| HWA217/pNZ9530 | 65 | 0.49 | 0.49 | |||||
| HWA217/pNZ9530/pNZmt1D | 88 | 0.53 | 0.02 | 0.01 | 0.56 | |||
a
Spectra were recorded for 46.2 min, except for the spectrum of NZ9010/pNZmt1D, which was recorded for 420 min. The values in parentheses are concentrations measured in the supernatants by HPLC.