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Purification of the R,S-IDS-cleaving C-N lyase from A. tumefaciens BY6
| Purification step | Vol (ml) | Total amt of protein (mg) | Sp acta (μmol of R,S-IDS min−1 [mg of protein]−1) | Yield (%) | Purification (fold) |
|---|---|---|---|---|---|
| Crude extract | 45 | 572 | 1.15 | 100 | 1 |
| Ultracentrifugation | 35 | 221 | 1.54 | 51.7 | 1.3 |
| Q-Sepharose HR 16/10 | 28 | 26.6 | 7.4 | 29.9 | 6.4 |
| Phenyl-Superose HR 10/10 | 11.5 | 3.7 | 16.2 | 30.5 | 14.1 |
The specific activity was determined by measuring the disappearance of R,S-IDS.
