TABLE 2.
Activity of the C-N lyase with various substratesa
| Substrate | Activity (μmol of S,S-IDS/min/mg) | Relative activity |
|---|---|---|
| R,S-IDS | 10.6 | 100 |
| S,S-IDS | 0.49 | 2.5 |
| R,R-IDS | 0 | 0 |
| Ca2+-R,R-IDS | 4.2 | 40 |
| Mg2+-R,S-IDS | 1.85 | 17 |
| Mn2+-R,S-IDS | 0.81 | 2.9 |
| Fe3+-R,S-IDS | 0 | No transformation |
| Fe2+-R,S-IDS | 0 | No transformation |
| Zn2+-R,S-IDS | 0 | No transformation |
| Cu2+-R,S-IDS | 0 | No transformation |
| S,S-EDDS | 0 | No transformation |
| Technical EDDS | 0 | No transformation |
| dl-Aspartic acid | 0 | No transformation |
| Nopaline | 0 | No transformation |
a
Enzyme assays were performed with 5 mM as described in Materials and Methods. The specific activities were determined by measuring the disappearance of the substrate.