TABLE 3.
Purification of the IDS-epimerase from A. tumefaciens BY6
| Purification step | Vol (ml) | Total amt of protein (mg) | Sp acta (μmol of R,R-IDS min−1 [mg of protein]−1) | Yield (%) | Purification (fold) |
|---|---|---|---|---|---|
| Crude extract | 46 | 1139 | |||
| Ultracentrifugation | 35 | 812 | |||
| Q-Sepharose HR 16/10 | 29.5 | 284.4 | 2.0 | 100.0 | 1.0 |
| Mono Q HR 16/10 | 11.5 | 89.7 | 5.0 | 79.4 | 2.5 |
| Phenyl-Superose HR 10/10 | 20 | 17 | 13 | 39.4 | 6.6 |
| Superdex 200 HiLoad 16/60 | 4 | 9.6 | 19 | 32.6 | 9.6 |
| Hydroxyapatite cHT5-I | 5 | 4.95 | 25 | 22.1 | 12.7 |
a
The specific activity was determined by measuring the disappearance of R,R-IDS.