TABLE 4.
Kinetic parameters of the IDS-epimerasea
Substrate | Km (mM) | Vmax (μmol/min) | kcat (s−1) | kcat/Km (s−1 M−1) |
---|---|---|---|---|
S,S-IDS | 1.5 (0.3) | 250 (20) | 139 | 93 |
R,R-IDS | 1.3 (0.3) | 130 (10) | 72 | 55 |
R,S-IDS | 1.1 (0.2) | 30 (5) | 17 | 15 |
The values in parentheses are standard deviations, and the pH was 8.5.