Figure 2.

Mice Lacking Foxp3⁺ Treg Cell Expression of Integrin β8 Display Normal T Cell Homeostasis

Control mice (Itgb8^fl/fl) and mice lacking expression of integrin β8 on Treg cells (Itgb8^fl/flfoxp3^YFP-Cre), aged 6–12 months old, were examined for potential disruption of T cell homeostasis and inflammation.

(A) Representative flow cytometry plots of thymic T cell populations analyzed via CD4/CD8 expression.

(B) Spleen, mesenteric LN (mLN), and large intestinal lamina propria (LILP) were examined for T cell cellularity.

(C) Spleen, mLN, LILP, and thymus were examined for percent Foxp3⁺ Treg cells.

(D and E) Percent CD4⁺ and CD8⁺ effector/memory T cell subsets (CD44^hiCD62^lo) (D) and percent intracellular IFN-γ and IL-17 expression by CD4⁺ T cells (E) was examined by flow cytometry.

(F) Colitic score and representative images of Itgb8^fl/fl and Itgb8^fl/flfoxp3^YFP-Cre mice colon.

(G) Representative histological sections of organs from Itgb8^fl/fl and Itgb8^fl/flfoxp3^YFP-Cre mice.

Data represent n = 4–5.