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Rarefaction curves for 16S rDNA clone libraries derived from DNA extracted from manure, the separator pit water, and the lagoon water using the Rarefaction Calculator (located at http://www.uga.edu/∼strata/software/) by Holland. Sequences were grouped into OTUs based on 97% or greater sequence similarity. Solid lines refer to the manure-derived library, dotted lines refer to the separator pit water-derived library, and dashed lines refer to the lagoon water-derived library.
