FIG. 2.

Phylogenetic relationship of the OTUs isolated from manure (M), the separator pit (SP), and lagoon water (L). The dendrogram was constructed using the neighbor-joining algorithm and the Jukes-Cantor distance estimation method. Bootstrap values (>70%) generated from 1,000 replicates are shown at the nodes. The scale bar represents substitutions per site. Phylum and order designations are indicated on the right. Due to space limitations, the two orders represented within the γ-Proteobacteria are not labeled. Acinetobacter johnsonii, M6, and Pseudomonas alcaligenes are members of the order Pseudomonadales; Achromatium, L8, L9, and Thiocapsa rosea are members of the order Chromatiales. Labels were added in Corel Draw 11 (Corel, Ottawa, Canada).