TABLE 3.
Ten most abundant OTUs from each library
| Source and OTU | Organism with or accession no. of most similar sequence in GenBank | % Similarity | No. of clonesa |
|---|---|---|---|
| Manure | |||
| M1 | Clostridium lituseburense | 97-99 | 31 |
| M2 | AF129864 | 95-97 | 26 |
| M3 | Ruminococcus bromii | 96-98 | 15 |
| M4 | AF018563 | 92-94 | 15 |
| M5 | AF018563 | 96-98 | 14 |
| M6 | Acinetobacter johnsonii | 98-100 | 14 |
| M7 | U81762 | 95-96 | 14 |
| M8 | Bacillus silvestris | 97 | 12 |
| M9 | AB009169 | 94-97 | 13 |
| M10 | Eubacterium tenue | 99 | 13 |
| Separator pit | |||
| SP1 | Clostridium lituseburense | 96-99 | 69 |
| SP2 | Eubacterium tenue | 97-100 | 31 |
| SP3 | Turicibacter sanguinis | 99-100 | 18 |
| SP4 | AF129864 | 95-96 | 15 |
| SP5 | Salinicoccus roseus | 96-99 | 14 |
| SP6 | Alkalibacterium olivoapovliticus | 92-93 | 13 |
| SP7 | Bacillus thermocloacae | 91-92 | 12 |
| SP8 | Alkalibacterium olivoapovliticus | 94-96 | 10 |
| SP9 | Clostridium celatum | 96-98 | 8 |
| SP10 | Lactosphaera pasteurii | 95-98 | 8 |
| Lagoon | |||
| L1 | Clostridium lituseburense | 97-99 | 99 |
| L2 | Eubacterium tenue | 98-100 | 47 |
| L3 | U81676 | 94-97 | 35 |
| L4 | AF087054 | 89 | 17 |
| L5 | Turicibacter sanguinis | 99-100 | 13 |
| L6 | Clostridium aminobutyricum | 93-94 | 13 |
| L7 | Streptococcus suis | 92-94 | 11 |
| L8 | Thiocapsa rosea | 95-96 | 11 |
| L9 | Achromatium sp. HK15 | 93-94 | 10 |
| L10 | U81715 | 94-96 | 9 |
a
Total numbers of clones in the top 10 for each location were as follows: 167 (33% of total) for manure, 198 (39% of total) for the separator pit, and 265 (63% of total) for the lagoon.