Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic | Reference |
|---|---|---|
| C. glutamicum | ||
| WT | wild type strain ATCC 13032 | [9] |
| ΔphoRS | deletion of the phoRS operon encoding the two component system PhoRS | [33] |
| ΔramB | Deletion of ramB encoding regulator of acetate metabolism B | [41] |
| E. coli | ||
| BL21(DE3) | ompT hsdS B(rB−mB−) gal dcm (DE3) | [64] |
| DH5α | F− thi-1 endA1 hsdr17(r−, m−) supE44 ΔblacU169 (ф80lacZΔM15) recA1 gyrA96 relA1 | [65] |
| Plasmids | ||
| pGEM-T | cloning vector | Promega, WI, USA |
| pET2 | promoter-probe vector | [54] |
| pET2-RF0 | pET2 with pstSCAB promoter fragment RF0 | This study |
| pET2-R0F0 | pET2 with pstSCAB promoterfragment R0F0 | This study |
| pET2-R1F0 | pET2 with pstSCAB promoter fragment R1F0 | This study |
| pET2-R2F0 | pET2 with pstSCAB promoter fragment R2F0 | This study |
| pET2-R3F0 | pET2 with pstSCAB promoter fragment R3F0 | This study |
| pET2-R0F1 | pET2 with pstSCAB promoter fragment R0F1 | This study |
| pET2-R0F2 | pET2 with pstSCAB promoter fragment R0F2 | This study |
| pET2-R0F3 | pET2 with pstSCAB promoter fragment R0F3 | This study |
| pET2-RcFc | pET2 with pstSCAB promoter fragment RcFc | This study |
| pET2-RcFm | pET2 with pstSCAB promoter fragment RcFm | This study |
| pET2-RmFc | pET2 with pstSCAB promoter fragment RmFc | This study |
| pET2-RmFm | pET2 with pstSCAB promoter fragment RmFm | This study |
| pET29-ramB-his | KanR; pET29-Histag derivative for over production of RamB with a C-terminal histidine tag | [41] |