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. 2015 May 29;15:113. doi: 10.1186/s12866-015-0437-1

Table 4.

Expression of various pstS promoter fragment cat fusions in C. glutamicum WT

Promoter fragment in transcriptional fusion Carbon source sp. act. of chloramphenicol acetyltransferase [μmol min−1 mg−1]a
0 min 90 minb
R0F0 Glucose 0.02 2.10
Acetate 0.01 0.22
RcFc Glucose <0.005 0.61
Acetate <0.005 0.10
RcFm Glucose <0.005 0.12
Acetate <0.005 0.01
RmFc Glucose <0.005 0.10
Acetate <0.005 0.01
RmFm Glucose <0.005 0.02
Acetate <0.005 <0.005

aAt least three determinations of two independent cultivations were performed. Average values are given with experimental imprecision < 20 %

bThe specific activity of chloramphenicol acetyltransferase was measured 0 and 90 min after a shift from Pi sufficient to Pi limiting conditions