Table 4.
Expression of various pstS promoter fragment cat fusions in C. glutamicum WT
Promoter fragment in transcriptional fusion | Carbon source | sp. act. of chloramphenicol acetyltransferase [μmol min−1 mg−1]a | |
---|---|---|---|
0 min | 90 minb | ||
R0F0 | Glucose | 0.02 | 2.10 |
Acetate | 0.01 | 0.22 | |
RcFc | Glucose | <0.005 | 0.61 |
Acetate | <0.005 | 0.10 | |
RcFm | Glucose | <0.005 | 0.12 |
Acetate | <0.005 | 0.01 | |
RmFc | Glucose | <0.005 | 0.10 |
Acetate | <0.005 | 0.01 | |
RmFm | Glucose | <0.005 | 0.02 |
Acetate | <0.005 | <0.005 |
aAt least three determinations of two independent cultivations were performed. Average values are given with experimental imprecision < 20 %
bThe specific activity of chloramphenicol acetyltransferase was measured 0 and 90 min after a shift from Pi sufficient to Pi limiting conditions