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Time-course analysis of hemolytic activity of the purified His-tagged CyaA-Hly toxin (10 μg/mL) against sheep erythrocytes (5 × 108 cells/mL). Inset, the plot of fractional hemolytic activity at 2 h, Y (PTM/1 − PTM), versus toxin concentration, [T]. Error bars indicate SEM from two independent experiments where each toxin concentration was done in triplicate.
