Table 1.

Hemolytic activity of CyaA-Hly toxins.

Toxin	Hemolytic activity b (%) ± SEM
CyaA-Hly cell lysate a	63.0 ± 3.0
6×His-tagged CyaA-Hly cell lysate a	66.0 ± 3.0

^a Soluble protein fraction of E. coli cell lysate (1 mg) containing ~10 μg CyaA-Hly toxins were incubated with sheep erythrocytes (5 × 10⁸ cells) in a total volume of 1 mL at 37 °C for 6 h; ^b Percent hemolysis was calculated as described in material and methods. The same amount of total proteins in the soluble fraction of E. coli lysate containing the pET17b vector gave <1% hemolysis (a negative control). The values were averaged from three independent experiments where each was performed in triplicates