TABLE 1.
Water samplesa and results of PCR and bioassay
| Sample type | Vol. (liters) | No. of oocysts added | No. of oocysts per liter | No. of samples | No. of positive samples/total no. tested (% positive)
|
|
|---|---|---|---|---|---|---|
| PCR | Bioassay | |||||
| DW | 100 | 1 × 103 | 10 | 10 | 10/10 (100) | 6/10 (60) |
| DW | 100 | 1 × 102 | 1 | 10 | 6/10 (60) | 3/10 (30) |
| PDW | 100 | 1 × 103 | 10 | 10 | 6/10 (60) | 5/10 (50) |
| PDW | 100 | 1 × 102 | 1 | 10 | 5/10 (50) | 2/10 (20) |
| RSW | 40 | 4 × 104 | 1,000 | 10 | 5/10 (50) | 3/10 (30) |
| RSW | 40 | 4 × 103 | 100 | 10 | 2/10 (20) | 0/10 (0) |
| RSW | 40 | 4 × 102 | 10 | 10 | 0/10 (0) | 0/10 (0) |
a
The samples were T. gondii oocyst seeded, GES capsule filtered and eluted.