Figure 4.

Cytokine secretions and mRNA level of T-cells cocultured with various tumor cell lines for 24 h. A. Supernatants were collected after 24 h of coculture and the secretion of cytokines were assessed by ELISA. Data showed representative results from at least 3 independent experiments. Unpaired, two-tailed Student’s t-test was used to calculate the p values. **, p < 0.001 using the unpaired Student’s t-test comparing the PD1-ACR T cells to the control T cells. B. Cytokine mRNA level in T-cells cocultured with various tumor cell lines for 24 h. C. Cytokine mRNA level/β-actin mRNA level in T-cells cocultured with various tumor cell lines for 24 h.