Figure 3.

GM-CSF enhanced the burn serum-diminished association of VE-cadherin and VEGFR2 (A). Low pAkt expression was observed after burn serum treatment, and the levels were increased to approximately normal levels when cells were pre-treated with GM-CSF (B). The number of apoptotic cells (shown in brown) increased after burn serum exposure, whereas the number of cells was unaltered by BSA treatment and attenuated by GM-CSF treatment (C) (Scale bar = 50 μm). GM-CSF increased cell viability in burn serum conditions as measured by the MTT assay (D). (*p < 0.05).