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. 2015 Mar 15;7(3):489–501.

Figure 5.

Figure 5

Effect of miR-29c overexpression suppress HUVECs migration and regulate IGF-1 and VEGF protein expression. A, B. Reintroduction of miR-29c depressed cells migration ability by cell migration assay. C, D. The down-regulated expression of miR-29c promoted the migration of HUVECs in a wound-healing assay. E, F. Immunocytochemistry assay to compare the activity of VEGF in miR-29c overexpressing and control cells. G, H. HUVECs that were transfected with miR-29c inhibitors and miR-29c mimics, respectively, were subjected to western blot for IGF-1, p-PI3K, p-AKT and VEGF protein expression. Representative WB showed that up-regulation of miR-29c obviously depressed protein expression of IGF1, p-PI3K, p-AKT and VEGF expression in HUVECs. (n = 3 repeats with similar results. *p < 0.05, **p < 0.01).