FIG. 7.

Examination of F. nucleatum in a four-species biofilm using FISH with Cy3-labled probes. (A to D) Confocal micrographs of four-species biofilms labeled with a F. nucleatum-specific probe (red) and counterstained with general nucleic acid stain Syto 9 (green). Areas of yellow indicate colocalization of both fluorescent markers. (A) One-hour sequentially inoculated biofilm. Bar, 40 μm. (B) Fourteen-hour sequentially inoculated biofilm. (C) One-hour coaggregate-inoculated biofilm. (D) Fourteen-hour coaggregate-inoculated biofilm. (E) Graph indicating the total biovolumes of the four-species biofilm (hatched bars) and biovolumes of F. nucleatum (Fn) (stippled bars) in sequentially and coaggregate-inoculated biofilms.