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. 2015 Mar 15;7(3):640–653.

Figure 3.

Figure 3

Differential expression of VEGFA and VEGF receptors in human osteosarcoma cell lines at the mRNA and protein level: A. Semi quantitative RT-PCR analysis of the expression of VEGFA isoform, VEGFR1 and VEGFR2 and GAPDH (reference) encoding RNA in total RNA isolated from parental low-metastatic MG63, HOS and HuO9 and respective metastatic MG63M6 and MG63M8, MNNG/HOS and 143B, and HuO9-H3, HuO9-M112 and HuO9-M132 sublines. B. Western blot analysis of VEGFA, VEGFR1 and VEGFR2 levels in total protein extracts of indicated cell lines, β-actin was used as a protein loading control.