Fig. 3.

Analysis of Aβ-specific immune responses in regular C57BL/6 mice upon vaccination with Aβ1-42. (a) Frequency of Aβ-specific IFNγ-producing splenocytes in immunized mice, as assessed by ELISPOT. Spleen cells (10⁶/wells) from mice immunized with either PBS/CpG/IFA or Aβ1-42 in CpG/IFA were restimulated in triplicate for 18 h with Aβ1-42 (40 μg/ml) or a panel of overlapping nonamer peptides (10 μg/ml) covering the full length of Aβ1-42. Results are presented as numbers of peptide-specific IFNγ-secreting cells per 10⁶ splenocytes, calculated after subtracting the mean number of spots obtained in the absence of peptide. (b, c) Aβ-specific antibody responses in Aβ1-42-immunized mice. Levels of anti-Aβ antibodies in the sera were determined by ELISA using Aβ-coated plates. Data are expressed as relative OD. Mean ± SD (two to four mice/group). Results are representative of two independent experiments