TABLE 1.
Primers and probes used for the quantification of nagAc-like, bacterial 16S rRNA, and luxE genes
| Target | Namea | Sequence (5′-3′) | Concn (nM) | Amplicon size (bp) | Reference or source |
|---|---|---|---|---|---|
| nagAc-like genes | nagAc-like-F | GGCTGTTTTGATGCAGA | 600 | 107 | This study |
| nagAc-like-R | GGGCCTACAAGTTCCA | 600 | |||
| nagAc-like-P | FAM-CCAGGCTGCATCACCCAGATA-BHQ-1 | 300 | |||
| luxE (Vibrio fischeri) | luxE-F | TATTGCACGTGATCGTCTTA | 500 | 84 | This study |
| luxE-R | TGCTCAAACCAATCTCCA | 500 | |||
| luxE-P | FAM-ACGCTTGCTTGGTTCTGTCAACTTC-BHQ-1 | 100 | |||
| 16S rRNA genes (bacteria) | 1055f | ATGGCTGTCGTCAGCT | 600 | 352 | 12 |
| 1392r | ACGGGCGGTGTGTAC | 600 | |||
| 16STaq1115-BHQ | FAM-CAACGAGCGCAACCC-BHQ-1 | 125 |
a
F, forward primer; R, reverse primer; P, TaqMan probe; FAM, 6-carboxyfluorescein; BHQ-1, Black Hole Quencher.