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. 2015 May 30;16(1):419. doi: 10.1186/s12864-015-1583-4

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© Rosinski-Chupinal et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Modifications in sRNA expression in response to an acidic stress. A. Detection of Srn015/csRNA10 and Srn070/csRNA12 by combined analysis of RNA-seq and dRNA-seq experiments is given as an example of sRNA detection B. sRNA expression was quantified using RNA-seq (triplicate experiments) on exponentially growing bacteria submitted or not to a 20 min-acid stress. Normalization and statistical assessment were performed using the EdgeR software. Expression values were arbitrarily fixed to 1 for samples grown at pH7. Ten sRNAs were shown differentially regulated (p <0.05 after correction for multiple-testing adjustment) in acidic conditions: 6 up-regulated, left panel and 4 down-regulated, right panel.