TABLE 2.
SNRs of microarray hybridization to probes with different sequence similarities
Probe sequence identity (%) | NRa
|
|||||
---|---|---|---|---|---|---|
gi151388b | gi151385 | gi5070351 | gi5824143 | gi5824146 | gi5824147 | |
100 | 8.5 ± 2.5 | 19.2 ± 5.2 | 20.6 ± 6.2 | 31.2 ± 12.3 | 25.5 ± 8.5 | 10.5 ± 3.8 |
98 | 3.7 ± 1.1 | 18.3 ± 3.6 | 10.2 ± 3.5 | 25.6 ± 8.5 | 16.2 ± 7.6 | 12.6 ± 4.2 |
96 | 4.9 ± 1.3 | 12.7 ± 1.3 | 11.8 ± 3.6 | 20.4 ± 9.3 | 11.3 ± 4.2 | 6.4 ± 3.2 |
94 | 0.9 ± 0.1d | 12.6 ± 2.3 | 10.4 ± 2.8 | 22.2 ± 8.9 | 12.1 ± 0.8 | 7.2 ± 2.1 |
92 | 0.2 ± 0.1 | 7.5 ± 3.2 | 6.3 ± 3.2 | 11.5 ± 6.2 | 8.2 ± 0.2 | 0.5 ± 0.2 |
90 | 0.2 ± 0.0 | 0.8 ± 0.3 | 1.2 ± 0.5 | 7.5 ± 2.5 | 1.5 ± 0.4 | 0.3 ± 0.1 |
88 | 0.1 ± 0.1 | 0.0 ± 0.1 | 0.2 ± 0.1 | 0.1 ± 0.0 | 0.3 ± 0.2 | 0.0 ± 0.0 |
86c | 0.1 ± 0.0 | 0.9 ± 0.3 | 0.3 ± 0.2 | 0.5 ± 0.3 | 0.1 ± 0.0 | 0.3 ± 0.1 |
The data represent mean values and standard deviations derived from three independent microarray slides, with two replicates on each slide.
gi151388, naphthalene dioxygenase large subunit; gi151385, naphthalene dioxygenase (reductase subunit); gi5070351, cis-1,2-dihydro-1,2-dihydroxynaphthalene dehydrogenase; gi5824143, alkane 1-monooxygenase; gi5824146, aldehyde dehydrogenase; gi5824147, alcohol dehydrogenase.
SNRs for probes of identities from 76 to 84% are all <0.3.
Numbers for threshold points above which SNR is >3 are in boldface.