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Purification steps of recombinant SSG
| Step | Vol (ml) | Total activity (U) | Total amt of protein (mg) | Sp ac (U/mg) | Yield (%) | Fold purifi- cation |
|---|---|---|---|---|---|---|
| Cell extraction | 450 | 936 | 1,345 | 0.696 | 100 | 1.00 |
| Heat treatment | 425 | 787 | 279 | 2.82 | 84 | 4.05 |
| Butyl-Sepharose | 25 | 150 | 13.13 | 11.43 | 16 | 16.4 |
| Superdex 200 HR 10/30 | 4 | 43.94 | 0.78 | 56.3 | 4.6 | 80.9 |
