TABLE 2.

Synthesis of phenol from benzene, catechol from phenol, and 1,2,3-THB from catechol by E. coli TG1 cells expressing wild-type T4MO, T3MO, and TOM^a

Enzyme	Phenol formation from benzene		Catechol formation from phenol		1,2,3-THB formation from catechol		Toluene oxidation rate (nmol/min/mg protein)
	Initial formation rate (nmol/min/mg of protein)	Maximum production (μM)	Initial formation rate (nmol/min/mg of protein)	Maximum production (μM)	Initial formation rate (nmol/min/mg protein)	Maximum production (μM)
T4MOb	19 ± 1.6	144 ± 47	13.6 ± 0.3	103 ± 10	2.5 ± 0.5	132 ± 22	10 ± 0.8
T3MOb	3 ± 1	122 ± 43	3.1 ± 0.3	119 ± 13	0.26 ± 0.09	73 ± 4	4 ± 0.6
TOMc	0.89 ± 0.07	27 ± 12	1.8 ± 0.5	140 ± 7	1.7 ± 0.3	103 ± 22	2.4 ± 0.3

^aAs determined on the basis of HPLC analysis, the means ± standard deviations of at least two independent results are shown. The initial benzene liquid concentration was 165 μM on the basis of a Henry's law constant of 0.22 (9) (a 400 μM concentration was added as if all the benzene was in the liquid phase), and the initial toluene concentration was 165 μM on the basis of a Henry's law constant of 0.27 (9) (a 455 μM concentration was added as if all the toluene was in the liquid phase).

^bProtein concentration, 0.24 mg of protein/ml·OD.

^cProtein concentration, 0.22 mg of protein/ml·OD.