TABLE 2.
Specific activities of some enzymes related to UDP-glucose metabolism in crude extracts from C. glutamicuma
| Strain | Induction with 1 mM IPTG | Enzyme | Sp act (pkat · mg of protein−1) |
|---|---|---|---|
| ΔmluI/pLPIgalU01 | + | UDP-glucose pyrophosphorylase | 18,910 |
| ΔmluI/pLPIgalU01 | − | UDP-glucose pyrophosphorylase | 212 |
| ΔmluI/pLPIgalU02 | − | UDP-glucose pyrophosphorylase | 178 |
| ΔmluI | − | UDP-glucose pyrophosphorylase | 25 |
| ΔmluI | − | Trehalose-6-P phosphatase | 206 |
| ΔmluI/pLPIgalUotsBA01 | + | UDP-glucose pyrophosphorylase | 9,808 |
| ΔmluI/pLPIgalUotsBA01 | + | Trehalose-6-P phosphatase | 243 |
| ΔmluI/pLPIgalUotsBA01 | − | UDP-glucose pyrophosphorylase | 201 |
| ΔmluI/pLPIgalUotsBA01 | − | Trehalose-6-P phosphatase | 367 |
| ΔmluI | − | Phosphoglucomutase | 1,330 |
| ΔmluI/pLPIgalU01 | + | Inorganic pyrophosphatase | 14,718 |
| ΔmluI/pLPIgalU01 | + | UDP-glucose-4-epimerase | 57 |
| ΔmluI/pLPIgalU01 | + | UDP-galactose:glucose-1-P uridylyltransferase | 36 |
a
The specific activities are the means of at least two independent measurements, and the standard deviation was always less than 10%. Extracts were prepared from cells grown in shake flasks with DMCG I and were induced for 6 h with 1 mM IPTG. The inducer was added when the culture reached an optical density at 600 nm of 0.3 to 0.4.