TABLE 5.
Comparison of inhibitory activities of two superior competitive-exclusion microorganisms (CEM) against L. monocytogenes in TSB and biofilms
| CEM strain |
L. monocytogenes count (log10 CFU/ml) in TSB at temp:
|
L. monocytogenes count (log10 CFU/cm2) in biofilm at temp:
|
||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 37°C
|
15°C
|
8°C
|
4°C
|
37°C
|
15°C
|
8°C
|
4°C
|
|||||||||
| Zero time | 24 h | Zero time | 14 days | Zero time | 21 days | Zero time | 28 days | Zero time | 24 h | Zero time | 28 days | Zero time | 28 days | Zero time | 35 days | |
| C-1-92 | 3.2 ± 0.3 | 0.7 ± 0.1 | 3.4 ± 0.2 | <0.7a | 3.4 ± 0.1 | 5.1 ± 0.2 | 3.0 ± 0.2 | 8.0 ± 0.4 | 2.9 ± 0.1 | <1.7b | 3.7 ± 0.1 | <1.7 | 3.7 ± 0.2 | <1.7 | 2.6 ± 0.1 | <1.7 |
| 152 | 3.4 ± 0.2 | 3.3 ± 0.2 | 3.3 ± 0.2 | 8.6 ± 0.3 | 3.2 ± 0.2 | 3.5 ± 0.3 | 3.4 ± 0.1 | 3.9 ± 0.1 | 2.9 ± 0.1 | <1.7 | 3.7 ± 0.1 | 6.5 ± 0.4 | 3.7 ± 0.2 | <1.7 | 2.6 ± 0.1 | 2.5 ± 0.0 |
| No CEM (control) | 3.3 ± 0.3 | 9.4 ± 0.5 | 3.4 ± 0.2 | 9.0 ± 0.5 | 3.3 ± 0.1 | 9.8 ± 0.7 | 3.4 ± 0.1 | 9.9 ± 0.3 | 2.9 ± 0.1 | 7.7 ± 0.2 | 3.7 ± 0.1 | 9.5 ± 0.3 | 3.7 ± 0.2 | 8.8 ± 0.3 | 2.6 ± 0.1 | 6.8 ± 0.5 |
a
Minimum detection limit by direct plating method (<0.7 log10 CFU/ml).
b
Minimum detection limit by direct plating method (<1.7 log10 CFU/cm2).