TABLE 5.
FISH results
| Parameter | Valueb
|
|||
|---|---|---|---|---|
| HF
|
LF
|
|||
| Ileum (n = 24) | Colon (n = 24) | Ileum (n = 24) | Colon (n = 24) | |
| Total cell counta | 2.1 ± 1.1 × 108 | 3.1 ± 2.4 × 1010 | 1.2 ± 1.2 × 108 | 2.3 ± 1.4 × 1010 |
| Total bacterial counta | 1.89 ± 1.4 × 108 | 2.7 ± 1.5 × 1010 | 1.17 ± 1.1 × 108 | 1.94 ± 0.9 × 1010 |
| Lactobacillus-enterococcusa | 1.5 ± 0.6 × 108d | 3.8 ± 2.1 × 108 | 0.4 ± 0.3 × 107 | 6.3 ± 3.1 × 108 |
| L. amylovorus-like (Probe, L-S-OTU171-a-A-0088)c | ||||
| No. of piglets colonized (%) | 20 (83) | 18 (75) | ND | ND |
| Median count | 1.3 × 108 | 3.7 × 109 | ND | ND |
| Range | 0.64-2.4 × 108 | 1.3-5.6 × 109 | ND | ND |
| L. reuteri- like (Probe, L-S-OTU173-a-A-0085)c | ||||
| No. of piglets colonized (%) | 18 (75) | 10 (41.2) | ND | 9 (37.5) |
| Median count | 7.7 × 107 | 5.8 × 107 | ND | 5.5 × 107 |
| Range | 4.4-12 × 107 | 5.3-6.2 × 107 | ND | 5.1-6.3 × 107 |
a
Results for total cell counts (DAPI staining), total bacterial counts (S-D-Bact-0338-a-A-17), and lactobacillus-enterococcus counts (S-G-Lab-0158-a-A-20).
b
Mean ± standard deviation (cells per gram). ND, no bacteria detected.
c
Specific counts for the probes L-S-OTU171-a-A-0088 (L. amylovorus-like) and L-S-OTU173-a-A-0085 (L. reuteri-like) for ileal- and colonic-lumen samples from piglets fed for 10 days on HF or LF diet.
d
Significant differences (P < 0.05) from the values compared (bold face).