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. 2004 Aug;24(15):6824–6836. doi: 10.1128/MCB.24.15.6824-6836.2004

FIG. 3.

FIG. 3.

GATA-2 and RARα interact through their zinc finger regions. (A) GST fusion proteins containing the indicated portions of GATA-2 were tested for the ability to bind Flag-tagged RARα present in 293T cell nuclear extract programmed with Flag-RARα expression plasmids. The first and last amino acids of the GATA-2 region present in the various GST fusions are indicated, and the abilities of the proteins to bind RARα are summarized schematically (top panel). Western blotting analysis of the pulldown materials with anti-Flag antibody is shown on the right (top), and Coomassie brilliant blue (CBB) staining is presented at the bottom to allow assessment of the quality and quantity of the various GST-GATA-2 proteins used. The values on the left indicate the positions of molecular size markers. (B) Reciprocal pulldown analyses in which various GST-RARα fusion proteins were analyzed for the ability to bind Flag-tagged GATA-2. BD, binding domain.