Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2004 Aug;24(15):6824–6836. doi: 10.1128/MCB.24.15.6824-6836.2004

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2004, American Society for Microbiology

PMC Copyright notice

FIG. 8. — Comparison of wild-type (wt) and mutant GATA-2 DNA-binding activities. (A) Western blot analysis of 293T cell extracts programmed by transient expression of Flag-tagged versions of wild-type GATA-2 and the mutant GATA-2 defective in the ability to interact with RARα. (B) EMSA analysis with radiolabeled oligonucleotide probe harboring a GATA motif from the murine CD34 enhancer region. Note the presence of a nonspecific DNA-binding activity that is variably seen in 293T cell nuclear extracts. (C) Dissociation assays with control 293T cell extracts or extracts programmed by expression of wild-type or mutant GATA-2. Note the presence of a nonspecific DNA-binding activity. (D) Quantitative analysis of the results presented in panel C. The percentage of probe bound by GATA-2 is plotted on a log scale versus time.