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Cell block specimen made from the pleural effusion presented as anaplastic lymphoma kinase (ALK) positive by: (a) Ventana immunohistochemistry; and (b) reverse transcription-polymerase chain reaction (RT-PCR). Epidermal growth factor receptor (EGFR) gene mutation negative by (c) amplification refractory mutation system (ARMS) polymerase chain reaction (PCR).
