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. 2015 May 29;5:10342. doi: 10.1038/srep10342

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Construction of binary vectors for genome editing in soybean. Cas9 fused with a single nuclear localization signal (NLS) is expressed with a Cauliflower mosaic virus 35s (CaMV 35s) promoter. Synthetic guide RNA (sgRNA) is derived using U6 promoters. (a) Arabidopsis thaliana U6-26 promoter (b) Glycine max U6-10 promoter. Sequences containing two BsaI sites are located between the U6 promoter and the sgRNA scaffold. These sequences can be easily replaced with a gene-specific sgRNA seed. LB: left border; RB: right border.