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. 2015 May 29;5:10342. doi: 10.1038/srep10342

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Copyright © 2015, Macmillan Publishers Limited

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Non-specific synthetic guide RNA (sgRNA) seeds in soybean. (6a) Distribution of non-specific sgRNA seeds and the number of their target genes. More than 1 million sgRNA seeds were associated with two target genes; approximately 100,000 sgRNA seeds were able to target three genes. sgRNA seeds having more than 100 target genes are not shown. (6b) Maximal gene coverage of non-specific sgRNA seeds. The non-specific sgRNA seeds were sorted by their target gene numbers before calculating the maximal gene coverage.