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. 2015 May 29;5:10627. doi: 10.1038/srep10627

Figure 2. Effects of Y27632 on the subcellular localization of MRTF-A/B in HAoECs.

Figure 2

HAoECs were cultured in HEC-C1 medium. For the last 12 h, they were treated with vehicle or 10 μM Y27632. (a) Whole cell lysates were subjected to IB analyses with the indicated antibodies. α↕tubulin was used as a loading control. (b and c) Cells were stained either with anti-MRTF-A antibody or anti-MRTF-B antibody (green), phalloidine-Alexa 568 (red), and Hoechst 33258 (blue). Representative images from at least three independent experiments are shown. Images were quantified as described in the legend for Fig. 1.