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. 2015 May 29;5:9774. doi: 10.1038/srep09774

Figure 3. CHIP negatively regulated cell migration and invasion in 786-O and OS-RC-2 cell lines.

Figure 3

(A) WB showed that twenty-four hours or forty-eight after transfection, CHIP protein was significantly overexpressed or knockdown in cancer cells, respectively. Actin was used as an internal control. (B) Cell migration assay was performed after reintroduction or knockdown of CHIP in 786-O and OS-RC-2 cells. The ability of cell migration was decreased after CHIP overexpression. In contrast, CHIP knockdown promoted cell migration. (C) Matrigel cell invasion assay was performed after reintroduction or knockdown of CHIP in 786-O and OS-RC-2 cells. The ability of cell invasion was decreased after CHIP overexpression while CHIP knockdown promoted cell invasion. The data are presented as mean ± s.e.m. for triplicate determinations. *** P < 0.001.