FIG. 1.

(i) Examples and analysis of identified DD products from PC5T EMT-6 mouse mammary tumor cells grown as monolayers (M) or spheroids (S). (ii to v) Northern blotting was carried out with total RNA (20 μg/lane) extracted from PC5T cells grown as monolayers (Mon) or spheroids (Sph). Probes were labeled with [³²P]dCTP. Sizes of bands were approximated by relative migration with respect to 18S and 28S bands. Ethidium bromide (EtBr) and ubiquitin mRNA are shown as loading controls. Grouped blots were generated by stripping and reprobing of the same filter.