FIG. 2.
Examples of Northern blotting analysis of DD products in a time course experiment on PC5T spheroid formation. Monolayers were trypsinized and plated on solid agarose for the times indicated. Total RNA was extracted, and approximately 20 μg was loaded per lane. Probes were labeled with [32P]dCTP. Sizes of bands were approximated by relative migration with respect to 18S and 28S bands. Ethidium bromide (EtBr) and ubiquitin mRNA are shown as loading controls. Grouped blots were generated by stripping and reprobing of the same filter.