FIG. 4.

RNS-mediated activation of JNK does not require new protein synthesis and occurs in a FasL-independent manner. C10 cells were exposed to 200 μM ONOO⁻ for 15 min and then chased for 2 h in the presence or absence of 10 μM CHX (A) or with 10-μg/ml anti-FasL antibody (B) or 250-ng/ml Fas-Fc (C). JNK activity was assayed via an in vitro kinase assay. In control experiments, cells were treated with 1-μg/ml M2 plus 100-ng/ml FasL (30 min) in the presence of either anti-FasL antibody (B) or Fas-Fc (C).