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. 2004 Aug;24(15):6763–6772. doi: 10.1128/MCB.24.15.6763-6772.2004

FIG. 5.

FIG. 5.

RNS-dependent activation of JNK occurs in FADD-dependent but caspase-independent manner. C10 cells were transfected with either pcDNA3 or FADD (80-205) (1.6 μg) in the presence of HA-JNK1 (0.4 μg) and then exposed to 200 μM ONOO− for 2 h (A) or 40 ppm of NO2 for 2 h (B). HA-JNK was immunoprecipitated, and JNK activity was assayed as described before. (C) C10 cells pretreated with 10 μM zVAD or IETD (30 min) were exposed to 200 μM ONOO− (2 h) or 1-μg/ml M2 plus 100-ng/ml FasL (30 min) prior to assessment of JNK activity. (D) ONOO−-induced cell death is caspase independent. C10 cells were pretreated with 10 μM zVAD or IETD (30 min) prior to exposure to 200 μM ONOO− (2 h) or 1-μg/ml M2 plus 100-ng/ml FasL (1 h) prior to assessment of cell death by using the MTT assay.