FIG. 7.

Blockade of PI3K inhibits TPO-induced MEIS1 expression. After 24 h of starvation, UT-7/TPO cells were treated with 20 μM LY294002 for 1 h and then incubated with TPO for 24 h, and nuclear protein and total cellular mRNA were prepared. Expression levels of MEIS1 were analyzed by real-time RT-PCR (A) and by Western blotting (B). (A) Each column represents the average MEIS1 level normalized to the GAPDH internal control ± standard deviation for three independent experiments. (B) Western blotting analysis of MEIS1 in the nuclear fraction. For an internal control the membrane was reprobed with an anti-TFIIH antibody.