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. 2004 Aug;24(15):6850–6860. doi: 10.1128/MCB.24.15.6850-6860.2004

FIG. 4.

FIG. 4.

The addition of the C-terminal domain diminishes ss DNA cleavage activity of the central domain in the presence and absence of RAG2. (A and B) DNA cleavage of a 5′-labeled ss WT 12-RSS (top) substrate by R1(CD) at 30 μg/ml (A) and R1(N+CD) at 2 μg/ml (B). To each protein, increasing concentrations of R1(CTD) from 1 to 30 μg/ml were added. The arrows indicate the lane in which R1(CTD) is equimolar with R1(CD) and 20-fold greater than R1(N+CD). (C) DNA cleavage activity on WT ss 12-RSS mediated by R1(CD) in the presence of R1(CTD) and/or core RAG2. Each lane contains R1(CD) at 30 μg/ml, lanes 2 and 4 contain equivalent concentrations of R1(CTD), and lanes 3 and 4 contain core RAG2 at 10 μg/ml. Products a, b, and c are labeled.