FIG. 5.

Comparison of the ds DNA cleavage activities of RAG1 fragments. Cleavage of a ds WT 12-RSS substrate is shown. The proteins (each at 10 μg/ml) are indicated above each lane. The reactions in lanes 1 to 6 were in 5 mM Mn²⁺ and those in lanes 7 to 10 were in 5 mM Mg²⁺. The hairpin and nicked products are labeled.