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. 2004 Aug;24(15):6811–6823. doi: 10.1128/MCB.24.15.6811-6823.2004

FIG. 6.

FIG. 6.

Effect of smu-2 mutation on expression of smu-1::HA. (A) Anti-HA immunofluorescence of SMU-1::HA in smu-2(+) and smu-2(mn416) genetic backgrounds. HA staining is dramatically reduced in smu-2 mutants, although staining is observed in some head neurons (arrow). (B) Western analysis of SMU-1::HA in smu-2(+) (lane 1) and smu-2(mn416) animals (lane 2). The major SMU-1::HA protein migrating at 78.5 kDa is markedly reduced in smu-2 mutants. Both the 78.5- and 86.5-kDa bands are specific to the smu-1::HA transgene since they do not appear in N2 animals (not shown). An antiactin antibody was used to monitor overall protein levels. (C) RT-PCR analysis of smu-1::HA transcripts in smu-2(+) (lane 1) and smu-2(mn416) (lane 2) animals. ama-1 was used as an internal control.