FIG. 1.

Localization of sense and antisense Stellate and Su(Ste) transcripts in testes. Sense and antisense RNA were detected by using single-stranded probes in whole testes of wild-type males (XY) and of males with a deletion of the bulk of Su(Ste) repeats (Xcry¹Y). DIG-labeled probes were visualized by using AP-coupled antibodies following color reaction with NBT/BCIP substrate. The segments shown in enlarged panels correspond to early (EPS) and mature (MPS) primary spermatocyte stages. In wild-type males, antisense transcripts are abundant in the nuclei of early primary spermatocytes, while in cry¹Y males the signal area is greatly decreased. In mature primary spermatocytes, antisense transcripts are detected as one or two sharp dots per nucleus. In cry¹Y males the signals are not visible in all nuclei. Sense RNA is detected only at the mature primary spermatocyte stage, as faint dots in nuclei of the wild-type males or as a strong diffuse signal in the cytoplasm of cry¹Y males.