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. 2004 Aug;24(15):6690–6700. doi: 10.1128/MCB.24.15.6690-6700.2004

FIG. 2.

FIG. 2.

Interaction and localization of C3G and E-cadherin in MCF7 cells. (A) Interaction between endogenous C3G and E-cadherin. MCF7 cells were incubated in low-calcium medium and then transferred to normal-calcium medium for the indicated times. Coimmunoprecipitation of endogenous C3G with E-cadherin was examined. Lanes: 2, no calcium switch; 1 and 3, low calcium; 4 to 8, switch from low calcium to normal calcium (lane 4, 15 min; lane 5, 30 min; lane 6, 1 h; lane 7, 2 h; lane 8, 4 h). (B) Localization of C3G during induction of cell-cell contacts. GFP-tagged C3G (full length) was transiently expressed in MCF7 cells, and the localization of C3G during calcium switch was analyzed by time-lapse images. Upper panel, phase contrast images. Lower panels, GFP-C3G. Arrows indicate the positions of transfected cells.