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Role of Rap1 in E-cadherin localization. After microinjection, MCF7 cells were subjected to a calcium switch, and the effect of expression of different molecules on the localization of E-cadherin (A and B, 30 min after calcium restoration) or ZO-1 (C, 60 min after calcium restoration) was examined by immunofluorescence. The following cDNAs were microinjected: (A and C) RapGAP; (B) C3G(N) (amino acids 1 to 357). Bars, 30 μm.
