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. 2004 Aug;24(15):6690–6700. doi: 10.1128/MCB.24.15.6690-6700.2004

FIG. 7.

FIG. 7.

Localization and role of Rap1 in N-cadherin-based cell-cell contacts in HEK293 cells. (A) N-cadherin but not E-cadherin is expressed in HEK293 cells. Cell lysates from HEK293 and MCF7 cells were examined for expression of E-cadherin and N-cadherin by Western blotting. (B) GFP-Rap1 is colocalized with N-cadherin at cell-cell contact sites in HEK293 cells after a calcium switch. GFP-Rap1 was transiently expressed in HEK293 cells, and the localization of GFP-Rap1 and N-cadherin was examined 1 h after calcium restoration. (C) The expression of RapGAP does not affect the localization of N-cadherin at cell-cell contact sites after a calcium switch. RapGAP was transiently expressed in HEK293 cells, and the localization of N-cadherin was examined 1 h after calcium restoration. Bar, 30 μm.