Skip to main content
. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: J Pharm Sci. 2015 Mar 5;104(5):1575–1591. doi: 10.1002/jps.24379

Figure 1.

Figure 1

Separation of nanometer-sized vs. micron-sized stirring-induced particles of mAb2 by low speed centrifugation. A) Schematic of experimental setup, and B) particle number and size distributions of the fractionated samples are shown in three different size ranges. The concentration of smaller particles (0.2–1.5 μm) for both supernatant and pellet was obtained by Resonant Mass Measurement (RMM; estimated RSD ~30 %, see text). The concentration of particles 2 μm to greater than 10 μm was obtained from light obscuration measurements (HIAC; estimated RSD 1%-8%, see text). The percentages were calculated by dividing the number of particles in either the supernatant or pellet by the total number of particles present in both supernatant and pellet at a particular size range.