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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: J Pharm Sci. 2015 Mar 5;104(5):1575–1591. doi: 10.1002/jps.24379

Figure 7.

Figure 7

Biophysical analysis of mAb2 solutions containing stirring-induced particles that were used as starting material for FACS separation. Control samples (unstressed and heat denatured) and two stirred samples (Stir-20h, Bottom) of mAb2 were characterized for morphology by SEM and TEM, composition by EDS, overall secondary structure by solution FTIR and FTIR microscopy, and surface hydrophobicity by extrinsic fluorescence using 1,8-ANS as indicated in this Figure. Representative TEM and SEM images are shown at two resolutions (the unstressed control had virtually no particles so no TEM and SEM images are shown). For FTIR analysis, shifts in two peaks for intramolecular beta sheet content (occurring between 1620 to 1640 cm−1 and 1690 to 1700 cm−1) were monitored in the second derivative FTIR spectra. The peak minima are shown for each sample (N=3 with the error bars representing one standard deviation).