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. 2014 Dec 17;1:14058. doi: 10.1038/mtm.2014.58

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Copyright © 2014 American Society of Gene & Cell Therapy

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/

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Effect of small interfering RNA (siRNA) treatment on electrotransfection efficiency. HEK293, HT29, and HCT116 cells were pretreated with either two different siRNA oligos against (a) CLTC, (b) CAV-1, or (c) Rab34, or with control siRNA with similar GC contents. At 48–72 hours post-treatment, the cells were electrotransfected, and the electrotransfection efficiency was quantified at 24 hours after incubation. All data were normalized by the matched controls. *P < 0.05 (n = 4–6, Mann–Whitney U-test).